Bruley-Rosset M, Hercend T, Martinez J, Rappaport H, Mathé G
J Natl Cancer Inst. 1981 Jun;66(6):1113-9. doi: 10.1093/jnci/66.6.1113.
Effects produced by long-term application of three immune modifiers (azimexon, retinoic acid, and tuftsin) on the depressed immune systems of 18-month-old inbred C57BL/6 female mice were investigated. The effect of each agent was examined on four cell types (cytotoxic T-cells, K-cells, NK cells, and macrophages) possibly involved in antitumor defenses and on the spontaneous tumor development that accompanied advancing age. Three substances chosen for this study appeared able to alter immune parameters, and each one displayed its own pattern of activity. Common to all three agents were an increase of age-depressed tumoricidal activity of peritoneal macrophages and no effect on the depressed NK activity of spleen cells. Retinoic acid increased splenic K-cell activity, already elevated in aged mice and unaffected by the other two agents. Cytotoxic T-cell activity, diminished by age, was stimulated considerably by retinoic acid and by tuftsin but only slightly by azimexon. Histopathologic studies revealed a decrease in the incidence of spontaneous tumors in the 3 treated groups. This decrease was statistically significant in the retinoic acid- and tuftsin-treated groups when compared with the incidence in untreated mice of the same age. Correlation of drug-induced modifications of the immune system with tumor incidence in aged mice was attempted.
研究了长期应用三种免疫调节剂（氮咪腙、视黄酸和促吞噬素）对18月龄近交C57BL/6雌性小鼠免疫抑制系统的影响。检测了每种药物对四种可能参与抗肿瘤防御的细胞类型（细胞毒性T细胞、K细胞、自然杀伤细胞和巨噬细胞）以及伴随年龄增长的自发肿瘤发生的影响。本研究选用的三种物质似乎能够改变免疫参数，且每种物质都表现出其自身的活性模式。所有三种药物的共同作用是增加了腹膜巨噬细胞随年龄下降的杀肿瘤活性，且对脾细胞下降的自然杀伤活性没有影响。视黄酸增加了脾K细胞的活性，而在老年小鼠中脾K细胞活性已经升高，且不受其他两种药物的影响。随年龄下降的细胞毒性T细胞活性受到视黄酸和促吞噬素的显著刺激，但仅受到氮咪腙的轻微刺激。组织病理学研究显示，三个治疗组的自发肿瘤发生率降低。与相同年龄未治疗小鼠的发生率相比，视黄酸和促吞噬素治疗组的这种降低具有统计学意义。尝试将药物诱导的免疫系统改变与老年小鼠的肿瘤发生率进行关联。
