Opstelten D, Stikker R, Deenen G J, Bos L, Nieuwenhuis P
Cell Tissue Res. 1981;218(1):59-73. doi: 10.1007/BF00210091.
The migration pattern of germinal center cells of the rabbit appendix was studied and compared with that of appendix dome cells, spleen cells, thymus cells and thoracic duct lymphocytes. To discriminate T- and B-cell migration pathways, normal or T-cell-depleted rabbits were used as donors. Cell suspensions were labeled in vitro with 3H-leucine followed by intravenous transfer. The migration of labeled cells in lymphoid organs was studied using autoradiography, particular attention being paid to the spleen of the recipient. B-cells from the appendix dome, spleen and thoracic-duct lymph migrate to primary follicles or the corona of secondary follicles via thymus-dependent areas of peripheral lymphoid organs. In contrast, a B-cell subpopulation from the germinal centers of the appendix migrates to the center of splenic primary follicles and into germinal centers. The migration of germinal center cells to splenic follicle centers is not enhanced by specific antigens. The migration properties of B-cells, possibly changing during differentiation, may be instrumental in the two types of immune reactions, i.e., plasma-cell reaction and germinal-center reaction.
研究了兔阑尾生发中心细胞的迁移模式，并将其与阑尾穹窿细胞、脾细胞、胸腺细胞和胸导管淋巴细胞的迁移模式进行了比较。为了区分T细胞和B细胞的迁移途径，使用正常或T细胞耗竭的兔子作为供体。细胞悬液在体外用3H-亮氨酸标记，然后进行静脉注射。使用放射自显影术研究标记细胞在淋巴器官中的迁移，特别关注受体的脾脏。来自阑尾穹窿、脾脏和胸导管淋巴的B细胞通过外周淋巴器官的胸腺依赖区迁移到初级滤泡或次级滤泡的冠部。相比之下，来自阑尾生发中心的B细胞亚群迁移到脾初级滤泡的中心并进入生发中心。特异性抗原不会增强生发中心细胞向脾滤泡中心的迁移。B细胞的迁移特性可能在分化过程中发生变化，这可能有助于两种免疫反应，即浆细胞反应和生发中心反应。
