Evans N T
Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Jun;39(6):597-605. doi: 10.1080/09553008114550721.
The effect of X-irradiation on small amounts of oxygen dissolved in 1 mM potassium chloride solution, tap water or Eagle's tissue culture medium has been followed with polarographic electrodes. Oxygen is removed from all these solutions down to concentrations corresponding to a few parts per million in the gas phase. Small amounts of hydrogen are also produced by irradiation and can be measured polarographically. Except in Eagle's medium, hydrogen peroxide is formed in the anoxic solutions, and molecular oxygen can be detected after irradiation when catalase is present in the solution. A non-volatile polarographically reducible substance is generated by irradiation of anoxic Eagle's medium. It is not decomposed by catalase but decays spontaneously in solution at a slow rate.
用极谱电极跟踪了X射线对溶解于1 mM氯化钾溶液、自来水或伊格尔组织培养基中的少量氧气的影响。氧气从所有这些溶液中被去除，直至气相中对应于百万分之几的浓度。辐照还会产生少量氢气，可用极谱法测量。除了在伊格尔培养基中，在缺氧溶液中会形成过氧化氢，当溶液中存在过氧化氢酶时，辐照后可检测到分子氧。缺氧的伊格尔培养基经辐照会产生一种非挥发性的可极谱还原物质。它不会被过氧化氢酶分解，但在溶液中会以缓慢的速率自发衰变。
