微生物培养物中过氧化氢积累的极谱测定法。
Polarographic assay of hydrogen peroxide accumulation in microbial cultures.
作者信息
Dempsey P M, O'Leary J, Condon S
出版信息
Appl Microbiol. 1975 Feb;29(2):170-4. doi: 10.1128/am.29.2.170-174.1975.
Abstract
A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 muM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria.
摘要
描述了一种通过使用极谱氧电极来测定低浓度过氧化氢的方法，该电极用于测量加入过氧化氢酶后释放到溶液中的氧气。样品无需预先处理即可直接在3分钟内进行检测。该方法能够检测低至7μM的过氧化氢浓度。事实证明，该方法对于检测乳酸菌分泌到牛奶中的过氧化氢非常有用。
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