Takada H, Hirachi Y, Hashizume H, Kotani S
Microbiol Immunol. 1981;25(3):317-26. doi: 10.1111/j.1348-0421.1981.tb00033.x.
The cytoplasmic membranes and a cytoplasmic fraction of Staphylococcus aureus L-forms increased the incorporation of [3H]thymidine by human lymphocytes in the presence of fetal bovine serum. Both fractions stimulated cord blood lymphocytes as well as adult peripheral lymphocytes, suggesting the possibility that the observed effect was not due to an antigen-specific reaction, but to an immunologically nonspecific action. The membrane mitogen(s) was resistant to trypsin, although it was partially solubilized by trypsin treatment. The mitogen(s) could not be extracted with a chloroform-methanol mixture (2:1, v/v), although the chloroform-methanol soluble fraction was strongly mitogenic to murine splenocytes. Human serum which was added to the assay system in place of fetal bovine serum definitely suppressed the mitogenic effect of both cytoplasmic membranes and the cytoplasmic fraction, especially the latter.
在胎牛血清存在的情况下，金黄色葡萄球菌L型的细胞质膜和细胞质组分增加了人淋巴细胞对[3H]胸苷的掺入。这两种组分均刺激脐血淋巴细胞以及成人外周淋巴细胞，这表明所观察到的效应可能不是由于抗原特异性反应，而是由于免疫非特异性作用。膜促有丝分裂原对胰蛋白酶有抗性，尽管经胰蛋白酶处理后它会部分溶解。促有丝分裂原不能用氯仿 - 甲醇混合物（2:1，v/v）提取，尽管氯仿 - 甲醇可溶级分对小鼠脾细胞有很强的促有丝分裂作用。用人类血清代替胎牛血清添加到测定系统中，确实抑制了细胞质膜和细胞质组分的促有丝分裂作用，尤其是后者。
