Iliushin V A, Maianskiĭ A N, Akatov A K
Zh Mikrobiol Epidemiol Immunobiol. 1983 Mar(3):36-40.
A fraction with a molecular weight of 16000-24000 was isolated from 5-day S. aureus broth culture grown in the dialysate of Martin-Hottinger broth. At a concentration of 2-15 micrograms/ml this fraction induced the nonspecific blast transformation of human blood lymphocytes with the stimulation index 99.6 +/- 15.2 (72-hour cultures). The mitogenic fraction was immunochemically inert and unrelated to the main part of biologically active components, resistant to heating at 100 degrees C for 10 minutes and to lyophilization, inactivated (by 84.2%-73.7%) by pronase and trypsin, not inactivated by specific immunosorption. Mitogenically active metabolites are regarded as a separate type of biologically active S. aureus products.
从在Martin-Hottinger肉汤透析液中培养5天的金黄色葡萄球菌肉汤培养物中分离出一种分子量为16000 - 24000的组分。该组分浓度为2 - 15微克/毫升时，可诱导人血淋巴细胞发生非特异性母细胞转化，刺激指数为99.6±15.2（72小时培养）。促有丝分裂组分在免疫化学上无活性，与生物活性成分的主要部分无关，耐100℃加热10分钟和冻干，被链霉蛋白酶和胰蛋白酶灭活（84.2% - 73.7%），不被特异性免疫吸附灭活。有丝分裂活性代谢产物被视为金黄色葡萄球菌生物活性产物的一种独立类型。
