Barot-Ciorbaru R, Brochier J, Miyawaki T, Preud'Homme J L, Petit J F, Bona C, Taniguchi N, Revillard J P
J Immunol. 1985 Nov;135(5):3277-83.
Nocardia-delipidated cell mitogen (NDCM), a particulate fraction prepared from Nocardia opaca, is able to stimulate the proliferation of small resting human B lymphocytes and their differentiation into Ig-secreting cells. This fraction contains two active structures: the cell wall peptidoglycan (PG) and a fraction (Cy I) derived from the cytoplasmic compartment. Treatment of insoluble PG with various bacteriolytic enzymes showed that the minimal structure required for mitogenic activity is more complex than that required for the differentiation of human lymphocytes. The mitogenic activity of cell wall fractions varies in different bacterial species; that prepared from N. opaca is the more potent. Both mitogenic structures of N. opaca induce higher responses in infant and adult PBL as compared to cord lymphocytes. The differentiation of B lymphocytes into Ig-secreting cells induced by PG fractions is T-dependent.
诺卡氏菌脱脂细胞促有丝分裂原（NDCM）是从不透明诺卡氏菌制备的颗粒部分，能够刺激静止的人小B淋巴细胞增殖并分化为分泌Ig的细胞。该部分包含两种活性结构：细胞壁肽聚糖（PG）和源自细胞质区室的一部分（Cy I）。用各种溶菌酶处理不溶性PG表明，促有丝分裂活性所需的最小结构比人淋巴细胞分化所需的结构更复杂。细胞壁部分的促有丝分裂活性在不同细菌种类中有所不同；从不透明诺卡氏菌制备的活性更强。与脐带血淋巴细胞相比，不透明诺卡氏菌的两种促有丝分裂结构在婴儿和成人外周血淋巴细胞中诱导出更高的反应。PG部分诱导的B淋巴细胞分化为分泌Ig的细胞是T细胞依赖性的。
