Fisher P B, Mufson R A, Weinstein I B, Little J B
Carcinogenesis. 1981;2(3):183-7. doi: 10.1093/carcin/2.3.183.
The polypeptide hormone epidermal growth factor (EGF) has been shown to enhance adenovirus type 5 transformation of a cloned culture of rat embryo cells (CREF) and X-ray or u.v.-light induced transformation of 10T1/2 mouse embryo cells. In both systems, the degree of enhancement was quantitatively similar to that observed in treated rats grown in the presence of the potent tumor promoting agent 12-O-tetradecanoyl-phorbol-13-acetate (TPA). An increase in viral transformation was also observed in cells continuously exposed to phorbol esters with known promoting activity on mouse skin, but not structurally related analogs, inactive or weakly active in the two-stage mouse skin carcinogenesis assay. In addition, the appearance of transformed foci was accelerated and colonies tended to be larger in cultures grown in the presence of EGF or TPA. The present studies suggest the possibility that EGF may function as an endogenous promoter of carcinogenesis and further indicates that in vitro cell transformation systems may prove useful in identifying such agents.
多肽激素表皮生长因子（EGF）已被证明可增强大鼠胚胎细胞克隆培养物（CREF）的5型腺病毒转化，以及10T1/2小鼠胚胎细胞的X射线或紫外线诱导转化。在这两个系统中，增强程度在数量上与在强效肿瘤促进剂12-O-十四烷酰佛波醇-13-乙酸酯（TPA）存在下生长的经处理大鼠中观察到的相似。在持续暴露于对小鼠皮肤具有已知促进活性的佛波酯的细胞中也观察到病毒转化增加，但在两阶段小鼠皮肤致癌试验中无活性或活性较弱的结构相关类似物则不会。此外，在存在EGF或TPA的培养物中，转化灶的出现加速，且集落往往更大。目前的研究表明EGF可能作为致癌作用的内源性促进剂发挥作用，进一步表明体外细胞转化系统可能有助于识别此类因子。
