淋巴细胞白血病、髓细胞白血病和毛细胞白血病的Fcγ结合蛋白的分子量分析
Molecular weight analysis of Fc gamma-binding proteins of lymphoid leukemia, myeloid leukemia, and hairy-cell leukemia.
作者信息
Stein H, Thoenes J, Klatt U, Gerdes J, Müller V, Havsteen B
出版信息
J Cancer Res Clin Oncol. 1981;101(1):75-80. doi: 10.1007/BF00405067.
The molecular weights of EDTA-mercaptoethanol-soluble Fc gamma-affined proteins isolated from chronic lymphocytic leukemia of the B type, prolymphocytic leukemia of the B type, chronic myeloid leukemia and hairy-cell leukemia were compared. SDS polyacrylamide gel electrophoresis of the Fc gamma-binding material obtained from all six cases of B type leukemia revealed a single peak with an apparent molecular weight of 28,000. The Fc gamma-affined material isolated from the cells of two cases of chronic myeloid leukemia showed two peaks, one with an apparent molecular weight of 42,600 and one with an apparent molecular weight of 18,800. The Fc gamma-affined material isolated from the cells of two cases of hairy-cell leukemia electrophoresed in the form of a closely spaced double peak. One component of the double peak had an apparent molecular weight of 28,000 and thus corresponds to the Fc gamma-binding material of leukemic B cells. The second component had a slightly lower molecular weight. The latter component is not present on either leukemic B cells or myeloid cells. The results indicate that the EDTA-mercaptoethanol-soluble Fc gamma-affined proteins of different types of cells differ in molecular weight, and thus in molecular structure.
对从B型慢性淋巴细胞白血病、B型幼淋巴细胞白血病、慢性粒细胞白血病和毛细胞白血病中分离出的乙二胺四乙酸-巯基乙醇可溶性Fcγ亲和蛋白的分子量进行了比较。对所有6例B型白血病获得的Fcγ结合物质进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，显示出一个单一峰，表观分子量为28,000。从2例慢性粒细胞白血病细胞中分离出的Fcγ亲和物质显示出两个峰，一个表观分子量为42,600，另一个表观分子量为18,800。从2例毛细胞白血病细胞中分离出的Fcγ亲和物质以紧密间隔的双峰形式电泳。双峰的一个成分表观分子量为28,000，因此与白血病B细胞的Fcγ结合物质相对应。第二个成分分子量略低。后一个成分在白血病B细胞或髓细胞上均不存在。结果表明，不同类型细胞的乙二胺四乙酸-巯基乙醇可溶性Fcγ亲和蛋白在分子量上存在差异，因而在分子结构上也存在差异。
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