人血清中的调节因子：对小鼠T细胞的影响。

Regulatory factors in human serum: effect on murine T cells.

作者信息

Skibiński G, Wieczorek Z, Zwoliński J

出版信息

Arch Immunol Ther Exp (Warsz). 1981;29(1):9-19.

Abstract

Effect of lymphocyte E rosette stimulatory and inhibitory factors from human serum on murine T cells was studied. It was shown that the stimulatory factor (a polypeptide, temperature sensitive, with a molecular weight about 20,000, isolated from healthy individuals) interacted, in vitro, with murine spleen T cell precursors and generated formation of Thy-1,2+ cells. The cells generated were also active in GvH reaction. The stimulatory factor induced, in vitro, formation of cortisone-resistant thymocytes. Unfractionated pool of spleen cells treated with the inhibitory factor (a beta-lipoprotein isolated from sera of humans with active pulmonary tuberculosis) showed lower number of Thy-1,2+ cells, and lower activity in GvH reaction. However, when the spleen cells were subsequently treated with the stimulatory factor, the initial number of Thy-1,2+ cells was restored. It was confirmed that the factors did not interact with the target cells in the presence of prostaglandin inhibitors.

摘要

研究了人血清中淋巴细胞E玫瑰花结刺激因子和抑制因子对小鼠T细胞的作用。结果表明，刺激因子（一种从健康个体中分离出的对温度敏感、分子量约为20,000的多肽）在体外与小鼠脾脏T细胞前体相互作用，并促使Thy-1,2+细胞形成。所产生的细胞在移植物抗宿主反应中也具有活性。刺激因子在体外诱导了对可的松有抗性的胸腺细胞的形成。用抑制因子（从活动性肺结核患者血清中分离出的一种β-脂蛋白）处理的未分级脾细胞池显示，Thy-1,2+细胞数量减少，移植物抗宿主反应活性降低。然而，当脾细胞随后用刺激因子处理时，Thy-1,2+细胞的初始数量得以恢复。已证实，在存在前列腺素抑制剂的情况下，这些因子不与靶细胞相互作用。