脐血淋巴细胞中结核菌素纯化蛋白衍生物反应性T细胞
Tuberculin purified protein derivative-reactive T cells in cord blood lymphocytes.
作者信息
Shiratsuchi H, Tsuyuguchi I
出版信息
Infect Immun. 1981 Sep;33(3):651-7. doi: 10.1128/iai.33.3.651-657.1981.
Abstract
Lymphocytes obtained from cord blood of newborn babies who were born of healthy mothers were studied in vitro for their responsiveness to purified protein derivative (PPD) of tuberculin. Cord blood lymphocytes proliferated in vitro by stimulation with PPD, despite wide variations in the results. Studies with fractionated lymphocytes revealed that PPD-responding cells belonged to E-rosetting, nylon wool-nonadherent T lymphocytes. Non-E-rosetting B lymphocytes alone did not proliferate at all after stimulation with PPD. In addition, bromodeoxyuridine and light treatment of in vitro PPD-stimulated lymphocytes eliminated the responsiveness to PPD. These results suggest that T lymphocytes do exist in cord blood and respond in vitro to stimulation with PPD. A possible role for PPD-reactive T lymphocytes in cell-mediated protective immunity in newborns is discussed.
摘要
对健康母亲所生新生儿脐带血中的淋巴细胞进行体外研究，以观察其对结核菌素纯蛋白衍生物（PPD）的反应性。尽管结果差异很大，但脐带血淋巴细胞经PPD刺激后在体外增殖。对分离的淋巴细胞进行的研究表明，对PPD有反应的细胞属于E花环形成、尼龙毛非黏附性T淋巴细胞。单独的非E花环形成B淋巴细胞经PPD刺激后根本不增殖。此外，用溴脱氧尿苷和光照处理体外PPD刺激的淋巴细胞消除了对PPD的反应性。这些结果表明脐带血中确实存在T淋巴细胞，并且在体外对PPD刺激有反应。本文讨论了PPD反应性T淋巴细胞在新生儿细胞介导的保护性免疫中的可能作用。
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