Ruuskanen O, Pittard W B, Miller K, Pierce G, Sorensen R U, Polmar S H
J Immunol. 1980 Jul;125(1):411-3.
Human cord blood mononuclear cells (MNC) were stimulated with antibiotic killed Staphylococcus aureus Cowan I bacteria (S. aureus) or pokeweed mitogen (PWM) and cultured for 6 days. Immunoglobulin synthesis and secretion was measured with the indirect protein A plaque-forming cell (PFC) assay. All 14 cord blood specimens stimulated with S. aureus produced PFC comparable to that measured in adult peripheral blood cultures. PWM induced only minimal responses in cord MNC whereas good responses were demonstrated in adult cell cultures. The majority of the cord blood immunoglobulin synthesizing cells produced IgM, however, significant production of IgA was also seen. These observations were confirmed by detection of intracytoplasmic immunoglobulin in plasma cells with the use of fluorescent anti-immunoglobulin antibodies.
用人脐血单个核细胞（MNC）与经抗生素灭活的金黄色葡萄球菌 Cowan I 菌（金黄色葡萄球菌）或商陆丝裂原（PWM）刺激，并培养 6 天。采用间接蛋白 A 噬斑形成细胞（PFC）试验检测免疫球蛋白的合成与分泌。所有 14 份经金黄色葡萄球菌刺激的脐血标本产生的 PFC 与成人外周血培养物中测得的相当。PWM 在脐血 MNC 中仅诱导出最小反应，而在成人细胞培养物中则表现出良好反应。大多数脐血免疫球蛋白合成细胞产生 IgM，但也可见到显著的 IgA 产生。通过使用荧光抗免疫球蛋白抗体检测浆细胞中的胞质内免疫球蛋白，证实了这些观察结果。
