Roholl P J, Leene W, Kapsenberg M L, Vos J G
J Immunol Methods. 1981;42(3):285-9. doi: 10.1016/0022-1759(81)90157-5.
Tannic acid as a prefixative for EM purposes was introduced by Futaesaku et al. (1972). The fixative creates conditions for enhancing electron density of different protein materials. By using a mixture of tannic acid and glutaraldehyde as prefixative, followed by a routine procedure of postfixation (OsO4) and poststaining (uranylacetate and lead citrate), membrane bound antibodies not conjugated with electron dense markers are made visible under the electron microscope.
富田作等人（1972年）引入了鞣酸作为用于电子显微镜目的的预固定剂。该固定剂为提高不同蛋白质材料的电子密度创造了条件。通过使用鞣酸和戊二醛的混合物作为预固定剂，随后进行常规的后固定（四氧化锇）和后染色（醋酸铀和柠檬酸铅）程序，未与电子致密标记物结合的膜结合抗体在电子显微镜下可见。
