Dillon S, Morad M
Science. 1981 Oct 23;214(4519):453-6. doi: 10.1126/science.6974891.
A rapid laser scanning system was developed to map the spread of excitation in amphibian and mammalian hearts stained with fluorescent dye. Isochronic maps of conduction were constructed by timing the upstroke of the optical action potential; 128 sites could be scanned in 4 milliseconds. The accuracy of this technique was verified by recording simultaneously from 16 unipolar electrodes placed in different areas of the heart. Conducted action potentials in normal frog heart propagated at 0.1 meter per second. Propagation of action potentials was also monitored in ischemic cat heart, in which both driven and arrhythmic action potential upstrokes could be tracked. The results suggest that this system is capable of scanning the normal and abnormal spread of electrical activity in the heart.
开发了一种快速激光扫描系统，用于绘制用荧光染料染色的两栖动物和哺乳动物心脏中兴奋的传播情况。通过对光学动作电位的上升支计时来构建等时传导图；4毫秒内可扫描128个位点。通过同时记录放置在心脏不同区域的16个单极电极来验证该技术的准确性。正常青蛙心脏中的传导动作电位以每秒0.1米的速度传播。还在缺血性猫心脏中监测动作电位的传播，在该心脏中可以追踪驱动性和心律失常性动作电位的上升支。结果表明，该系统能够扫描心脏中电活动的正常和异常传播情况。
