Bowmaster T A, Davis C C, Krauthamer V
Electrical Engineering Department, University of Maryland, College Park 20742.
Biochim Biophys Acta. 1991 Jan 10;1091(1):9-14. doi: 10.1016/0167-4889(91)90214-i.
An optical probe capable of detecting intracellular potential changes in individual cells, in vitro, which has the potential for in vivo applications, has been developed. A single-mode optical fiber directs laser light onto cells stained with the voltage-sensitive fluorescent dye, WW781 and also returns part of the resulting fluorescence to a detection system. Frog cardiac cells in vitro were used in these initial experiments. The fractional change in fluorescent intensity of 10(-3) for a 50 mV shift in transmembrane potential obtained from a heart immobilized in zero calcium Ringer's solution is comparable to that reported for other optical methods. For hearts in normal calcium Ringer's solutions, very large reproducible motion related artifacts were detected.
已开发出一种能够在体外检测单个细胞内电位变化的光学探针，该探针具有体内应用潜力。一根单模光纤将激光导向用电压敏感染料WW781染色的细胞，并将产生的部分荧光返回检测系统。这些初始实验使用了体外培养的青蛙心脏细胞。在零钙林格氏液中固定的心脏，跨膜电位发生50 mV偏移时，荧光强度的分数变化为10(-3)，这与其他光学方法报道的结果相当。对于处于正常钙林格氏液中的心脏，检测到了与运动相关的非常大的可重复伪影。
