Hammarström S, Hellström U, Perlmann P, Dillner M L
J Exp Med. 1973 Nov 1;138(5):1270-5. doi: 10.1084/jem.138.5.1270.
Neuraminidase treatment of human peripheral blood lymphocytes uncovers cell surface receptors that bind purified A hemagglutinin from the snail Helix pomatia. No hemagglutinin was bound to untreated lymphocytes. Binding studies with (125)I-labeled hemagglutinin suggested that the number of receptors on neuraminidase-treated lymphocytes was approximately 1.10(6)/cell. The apparent association constant for hemagglutinin binding to lymphocytes, as calculated from Scatchard's plots, was 5-7.10(8) liters/mol. Immunofluorescent staining with FITC-conjugated hemagglutinin gave positive reactions with approximately 60% of the lymphocytes from normal donors. Positive staining was inversely related to the number of lymphocytes with Fc or complement receptors or with surface immunoglobulin, thus suggesting that See PDF for Structure the lymphocytes with receptors for Helix pomatia A hemagglutinin are T cells. Cell fractionation on columns charged with hemagglutinin indicate that these receptors may be used for separating subpopulations of human peripheral lymphocytes.
用神经氨酸酶处理人外周血淋巴细胞可揭示出能结合来自蜗牛（Helix pomatia）的纯化A血凝素的细胞表面受体。未处理的淋巴细胞不结合血凝素。用（125）I标记的血凝素进行的结合研究表明，经神经氨酸酶处理的淋巴细胞上受体的数量约为1.10（6）/细胞。根据Scatchard图计算，血凝素与淋巴细胞结合的表观缔合常数为5 - 7.10（8）升/摩尔。用异硫氰酸荧光素（FITC）偶联的血凝素进行免疫荧光染色，正常供体约60%的淋巴细胞呈阳性反应。阳性染色与具有Fc或补体受体或表面免疫球蛋白的淋巴细胞数量呈负相关，因此表明具有蜗牛A血凝素受体的淋巴细胞是T细胞。在装有血凝素的柱上进行细胞分级分离表明，这些受体可用于分离人外周淋巴细胞亚群。
