Nanishi F, Nomoto K, Taniguchi K, Kubo C, Takeya K
Jpn J Exp Med. 1981 Feb;51(1):9-16.
Specific cytotoxicity against histocompatibility antigens of a parental strain was induced in the spleens of sublethally irradiated F1 mice after intravenous transfer of lymphoid cells from another parental strain. Such cytotoxicity reached the highest level at 10 days after the transfer of 3 to 5 X 10(7) spleen cells and was ascribed to be T lymphocytes of donor parental origin. Secondary response of the generation of cytotoxicity was compared among in vivo rechallenge, in vitro MLC and transfer to irradiated F1 mice. Cytotoxicity was not so augmented by in vivo rechallenge. It may be attributable to early elimination of grafts. In in vitro MLC and transfer to irradiated F1 mice, the generation of cytotoxicity appeared earlier with higher magnitudes as compared to primary response. The transfer system to irradiated F1 recipients appears to be more suitable for analyses of the generation of cytotoxic T lymphocytes from memory cells than in vivo rechallenge.
在亚致死剂量照射的F1小鼠脾脏中，静脉注射来自另一亲本品系的淋巴细胞后，可诱导出针对亲本品系组织相容性抗原的特异性细胞毒性。在注射3至5×10⁷个脾细胞后的第10天，这种细胞毒性达到最高水平，且被认为归因于供体亲本来源的T淋巴细胞。对体内再次攻击、体外混合淋巴细胞培养（MLC）以及转移至受照射F1小鼠中细胞毒性产生的二次反应进行了比较。体内再次攻击并未使细胞毒性显著增强。这可能归因于移植物的早期清除。在体外MLC和转移至受照射F1小鼠的情况下，与初次反应相比，细胞毒性的产生出现得更早且强度更高。对于分析记忆细胞产生细胞毒性T淋巴细胞而言，转移至受照射F1受体的系统似乎比体内再次攻击更适合。
