Basso G, Semenzato G, Cocito M G, Pezzutto A, Agostini C, Zanesco L
Thymus. 1981 Nov;3(4-5):195-201.
Three subpopulations of human T lymphocytes (TG, TM, and Tnon-G/non-M cells) were evaluated by means of six different cytochemical reactions. Elevated staining activity was demonstrated in all the populations when stained for acid phosphatase (AcP), beta-glucuronidase (BG) and N-acetyl beta-glucosaminidase (NABG). While the majority of TM cells displayed an intense acid alpha-naphthyl acetate esterase (ANAE) positivity, only a smaller proportion of the Tnon-G/non-M exhibited a positive reaction and a much lower activity was found in the TG group. TM cells were also alpha-naphthyl acetate esterase (NAE)-and alpha-naphthyl butyrate esterase (NBE)-positive, showing a dot-like pattern. These reactions were present in a lower proportion in the Tnon-G/non-M population and nearly completely absent in the TG cells. The present study identifies different cytochemical phenotypes for the different T-lymphocyte subsets and the analysis of these phenotypes may indicate that the TM is the most cytochemically differentiated subpopulation.
通过六种不同的细胞化学反应对人类T淋巴细胞的三个亚群(TG、TM和T非G/非M细胞)进行了评估。当用酸性磷酸酶(AcP)、β-葡萄糖醛酸酶(BG)和N-乙酰-β-氨基葡萄糖苷酶(NABG)染色时,所有亚群均显示出增强的染色活性。虽然大多数TM细胞表现出强烈的酸性α-萘乙酸酯酶(ANAE)阳性,但只有较小比例的T非G/非M细胞呈现阳性反应,而TG组的活性则低得多。TM细胞对α-萘乙酸酯酶(NAE)和α-萘丁酸酯酶(NBE)也呈阳性,呈现点状模式。这些反应在T非G/非M亚群中的比例较低,在TG细胞中几乎完全不存在。本研究确定了不同T淋巴细胞亚群的不同细胞化学表型,对这些表型的分析可能表明TM是细胞化学分化程度最高的亚群。