人类淋巴细胞中唾液酸的性质。
The nature of sialic acids in human lymphocytes.
作者信息
Kamerling J P, Makovitzky J, Schauer R, Vliegenthart J F, Wember M
出版信息
Biochim Biophys Acta. 1982 Feb 2;714(2):351-5. doi: 10.1016/0304-4165(82)90344-0.
PMID:6976798
Abstract
Analysis of the sialic acids obtained by mild acid hydrolysis of B lymphocytes reveals the presence of N-acetylneuraminic acid and 9-O-acetyl-N-acetylneuraminic acid. For T lymphocytes only N-acetylneuraminic acid has been demonstrated to occur. The applied methods include quantitative colorimetry, thin-layer chromatography and combined gas-liquid chromatography-mass spectrometry.
摘要
对通过B淋巴细胞温和酸水解获得的唾液酸进行分析，结果显示存在N-乙酰神经氨酸和9-O-乙酰-N-乙酰神经氨酸。对于T淋巴细胞，仅证实存在N-乙酰神经氨酸。所应用的方法包括定量比色法、薄层色谱法以及气液色谱-质谱联用分析法。
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