Valesini G, Picardo M, Pastore R, Pivetti P, Serafini U
Clin Exp Immunol. 1981 Jun;44(3):522-7.
The C1q-binding assay was performed on 30 sera from patients with Behçet's syndrome and circulating immune complexes were found in 46%. Circulating immune complexes were isolated and purified from the sera of two patients by immunoadsorption on a column of polymethylmetacrylate beads coated with C1q and then labelled with 125I. In double immunodiffusion these purified immune complexes were found to contain IgG, C1q, C1s and C3. Anti-IgG activity was not detectable in the purified immune complexes while specific cross-reactivity was found in a solid-phase radioimmunoassay with the majority of the sera of behçet's syndrome.
对30例白塞病患者的血清进行了C1q结合试验，发现46%的患者存在循环免疫复合物。通过在包被有C1q的聚甲基丙烯酸甲酯珠柱上进行免疫吸附，从两名患者的血清中分离并纯化出循环免疫复合物，然后用125I进行标记。在双向免疫扩散试验中，发现这些纯化的免疫复合物含有IgG、C1q、C1s和C3。在纯化的免疫复合物中未检测到抗IgG活性，而在固相放射免疫测定中，发现白塞病综合征大多数患者的血清存在特异性交叉反应。
