Puchelle E, Zahm J M, Sadoul P
Am J Physiol. 1982 Jan;242(1):C31-5. doi: 10.1152/ajpcell.1982.242.1.C31.
The depleted frog palate epithelium is extensively used as a model for studying the transport velocity of mucus. It is speculated that the epithelium can be depleted of endogenous gel mucus without affecting the ciliary frequency, provided the sol phase where the cilia beat remains present. To control the stability of the ciliary frequency during the measurement of mucous transport velocity, we have used a photoelectric method for measuring mucociliary frequency and have carried out experiments in excised frog palates in different areas and at successive stages of depletion. From the distribution function of the mucociliary frequency obtained by the fast Fourier transform analysis, we have calculated the mean frequency (Fm) and the dispersion of the spectrum defined as spectrum width (Sw). Fm and Sw are similar in different areas throughout the zone selected for mucous transport velocity measurements. A significant decrease in Fm (21%) and parallel increase in Sw (22.8%) are observed at the stage of depletion, but the values return to normal when a drop of frog's mucus is added. These results suggest that the depleted frog palate is a good model for studying the in vitro transport rate of mucus and investigating the effects of mucus on ciliary frequency.
耗尽黏液的蛙腭上皮被广泛用作研究黏液运输速度的模型。据推测，只要纤毛摆动的溶胶相仍然存在，上皮中的内源性凝胶黏液就可以被耗尽而不影响纤毛频率。为了在测量黏液运输速度期间控制纤毛频率的稳定性，我们使用了一种光电方法来测量黏液纤毛频率，并在不同区域和耗尽的连续阶段对离体蛙腭进行了实验。通过快速傅里叶变换分析获得黏液纤毛频率的分布函数后，我们计算了平均频率（Fm）和定义为频谱宽度（Sw）的频谱离散度。在为黏液运输速度测量所选区域的不同部位，Fm和Sw相似。在耗尽阶段观察到Fm显著降低（21%），Sw平行增加（22.8%），但当加入一滴蛙黏液时，这些值恢复正常。这些结果表明，耗尽黏液的蛙腭是研究黏液体外运输速率和研究黏液对纤毛频率影响的良好模型。
