Newton I A, Metral J, Hudry-Clergeon G, Berthier R, Lajmanovich A, Schweitzer A, Chagnon E, Hollard D
Exp Hematol. 1982 Feb;10(2):196-205.
Conditioned media prepared using human placenta, spleen, bone marrow and peripheral blood leucocytes revealed a common pattern of two distinct species of colony-stimulating factors (CSF) separable by gel filtration. The peak of greatest activity, active against both human and mouse marrow, had an apparent molecular weight (MW) of 24,000-28,000 Daltons. A peak of low activity detected only against mouse marrow had an apparent MW of approximately 150,000 Daltons. The type of progenitor cells stimulated by the crude medium, by the low MW CSF species, and by the high MW species were similar for the four conditioned media despite their different origins. No difference was found in either the MW of the human active CSF present or the type of progenitor cells stimulated by media conditioned with cells of leukemic origin.
使用人胎盘、脾脏、骨髓和外周血白细胞制备的条件培养基显示出一种常见模式，即通过凝胶过滤可分离出两种不同的集落刺激因子（CSF）。活性最高的峰，对人和小鼠骨髓均有活性，其表观分子量（MW）为24,000 - 28,000道尔顿。仅对小鼠骨髓检测到的低活性峰，其表观MW约为150,000道尔顿。尽管四种条件培养基来源不同，但粗培养基、低MW CSF种类和高MW种类所刺激的祖细胞类型相似。在存在的人活性CSF的MW或由白血病来源细胞制备的培养基所刺激的祖细胞类型方面均未发现差异。
