Neumeier R, Maurer H R
Exp Hematol. 1980 Jul;8(6):728-36.
Medium conditioned by bovine lung tissue (BLCM) contains a factor (CSFBL) that can stimulate either murine or human bone marrow cells to form colonies. CSFBL was partially purified by gel filtration and DEAE-cellulose chromatography. The specific activity was determined using both target systems. In all stages of purification the specific activity on human bone marrow was about 2 times greater than on mouse bone marrow. Determination of the molecular weight of CSFBL by gel filtration and comparison with mouse lung CSF (CSFML) revealed that both are similar in size, having an apparent molecular weight of 29,000 d. CSFBL stimulates only granulocyte colonies, neither macrophage nor mixed colonies could be detected in either bone marrow assay. These results and the ease of preparation recommend BLCM as a suitable starting for large scale preparation of CSF active on human cells.
由牛肺组织制备的条件培养基（BLCM）含有一种因子（CSFBL），它能刺激小鼠或人的骨髓细胞形成集落。通过凝胶过滤和DEAE-纤维素色谱法对CSFBL进行了部分纯化。使用两种靶细胞系统测定了比活性。在纯化的各个阶段，CSFBL对人骨髓的比活性比对小鼠骨髓的比活性高约2倍。通过凝胶过滤法测定CSFBL的分子量，并与小鼠肺集落刺激因子（CSFML）进行比较，结果显示二者大小相似，表观分子量均为29,000道尔顿。CSFBL仅刺激粒细胞集落形成，在两种骨髓检测中均未检测到巨噬细胞集落或混合集落。这些结果以及其制备的简便性使得BLCM成为大规模制备对人细胞有活性的集落刺激因子的合适起始材料。
