Williams N, Jackson H
Exp Hematol. 1977 Nov;5(6):523-34.
Granulocyte and macrophage progenitor cells which clone in semi-solid agar cultures were characerized by separating murine bone marrow cells by equilibrium density centrifugation. These in vitro colony forming cells (CFU-c), when induced by stimuli in mouse lung-conditioned medium, were found to be heterogeneous in their density properties. Differences were found in the dose responsiveness of two major subpopulations of these progenitor cells. One population (modal density 1.074 gcm-3) responded in an identical manner to stimuli present in mouse lung-conditioned medium and pregnant mouse uterus extract. A second population (modal density 1.070 gcm-3) was found to be markedly different in its dose responsiveness to mouse lung-conditioned medium, from the other subset (1.074 gcm-3). The cloning of the low density progenitor cells and the observed dose responsiveness was found to correlate with an activity present in a stimulus in mouse lung-conditioned medium but was not identical to the stimulus in pregnant mouse uterus extract. No subpopulation, however, gave exclusively granulocyte or macrophage colonies.
通过平衡密度离心法分离小鼠骨髓细胞，对在半固体琼脂培养中克隆的粒细胞和巨噬细胞祖细胞进行了表征。这些体外集落形成细胞（CFU-c）在小鼠肺条件培养基中的刺激下，其密度特性存在异质性。在这些祖细胞的两个主要亚群的剂量反应性方面发现了差异。一个群体（模态密度为1.074 g/cm³）对小鼠肺条件培养基和怀孕小鼠子宫提取物中存在的刺激以相同方式作出反应。发现第二个群体（模态密度为1.070 g/cm³）对小鼠肺条件培养基的剂量反应性与另一个亚群（1.074 g/cm³）明显不同。低密度祖细胞的克隆以及观察到的剂量反应性与小鼠肺条件培养基中一种刺激物的活性相关，但与怀孕小鼠子宫提取物中的刺激物不同。然而，没有一个亚群仅产生粒细胞或巨噬细胞集落。
