Paskin N, Mayer R J
Biochem J. 1978 Jul 15;174(1):153-161. doi: 10.1042/bj1740153.
A method for the calculation of rate constants of degradation of a specific protein during a period of change in protein amount is described. The calculation uses measurements of rates of synthesis and amount of the protein, together with one estimate of the protein half-life. The method is demonstrated by using data from measurements made on the accumulation of fatty acid synthetase in hormonally treated explants of mammary gland from mid-pregnant rabbits, and data from other authors. These calculations demonstrate a regulated programme of co-ordinated changes in rates of synthesis and degradation during a period of change in protein mass. The role of such changes in protein degradation during protein accumulation is discussed.
本文描述了一种计算特定蛋白质在蛋白质含量变化期间降解速率常数的方法。该计算使用了蛋白质合成速率和含量的测量值,以及蛋白质半衰期的一个估计值。通过使用来自对中孕兔乳腺激素处理外植体中脂肪酸合成酶积累的测量数据以及其他作者的数据,对该方法进行了验证。这些计算表明,在蛋白质质量变化期间,合成和降解速率存在协调变化的调控程序。本文还讨论了蛋白质积累过程中蛋白质降解这种变化的作用。