一种分析蛋白质周转特征的方法。蛋白质降解速率的间接估计。
A method for the analysis of protein turnover characteristics. Indirect estimation of rates of protein degradation.
作者信息
Paskin N, Mayer R J
出版信息
Biochem J. 1978 Jul 15;174(1):153-161. doi: 10.1042/bj1740153.
Abstract
A method for the calculation of rate constants of degradation of a specific protein during a period of change in protein amount is described. The calculation uses measurements of rates of synthesis and amount of the protein, together with one estimate of the protein half-life. The method is demonstrated by using data from measurements made on the accumulation of fatty acid synthetase in hormonally treated explants of mammary gland from mid-pregnant rabbits, and data from other authors. These calculations demonstrate a regulated programme of co-ordinated changes in rates of synthesis and degradation during a period of change in protein mass. The role of such changes in protein degradation during protein accumulation is discussed.
摘要
本文描述了一种计算特定蛋白质在蛋白质含量变化期间降解速率常数的方法。该计算使用了蛋白质合成速率和含量的测量值，以及蛋白质半衰期的一个估计值。通过使用来自对中孕兔乳腺激素处理外植体中脂肪酸合成酶积累的测量数据以及其他作者的数据，对该方法进行了验证。这些计算表明，在蛋白质质量变化期间，合成和降解速率存在协调变化的调控程序。本文还讨论了蛋白质积累过程中蛋白质降解这种变化的作用。
相似文献
1
A method for the analysis of protein turnover characteristics. Indirect estimation of rates of protein degradation.一种分析蛋白质周转特征的方法。蛋白质降解速率的间接估计。
Biochem J. 1978 Jul 15;174(1):153-161. doi: 10.1042/bj1740153.
2
Regulation of enzyme turnover during tissue differention. Studies on the effects of hormones on the turnover of fatty acid synthetase in rabbit mammary gland in organ culture.组织分化过程中酶周转的调节。关于激素对器官培养的兔乳腺中脂肪酸合成酶周转影响的研究。
Biochem J. 1975 May;148(2):309-20. doi: 10.1042/bj1480309.
3
Regulation of enzyme turnover during tissue differentiation. Interactions of insulin, prolactin and cortisol in controlling the turnover of fatty acid synthetase in rabbit mammary gland in organ culture.组织分化过程中酶周转的调节。胰岛素、催乳素和皮质醇在器官培养中对兔乳腺脂肪酸合成酶周转的控制中的相互作用。
Biochem J. 1976 Feb 15;154(2):359-70. doi: 10.1042/bj1540359.
4
Protein degradation during terminal cytodifferentiation. Studies on mammary gland in organ culture.终末细胞分化过程中的蛋白质降解。器官培养中乳腺的研究。
Biochem J. 1980 Oct 15;192(1):311-20. doi: 10.1042/bj1920311.
5
Coordination of protein synthesis and degradation.蛋白质合成与降解的协调
Ciba Found Symp. 1979(75):253-72. doi: 10.1002/9780470720585.ch16.
6
Characteristics of protein degradation in liver and mammary gland.肝脏和乳腺中蛋白质降解的特征。
Acta Biol Med Ger. 1981;40(10-11):1397-406.
7
Co-ordinate changes in enzymes of fatty acid synthesis, activation and esterification in rabbit mammary gland druing pregnancy and lactation.家兔乳腺在妊娠和泌乳期间脂肪酸合成、活化及酯化过程中酶的协同变化。
Biochem J. 1977 Feb 15;162(2):445-50. doi: 10.1042/bj1620445.
8
Protein synthesis and degradation during development.发育过程中的蛋白质合成与降解。
Crit Rev Food Sci Nutr. 1982;16(2):149-63. doi: 10.1080/10408398209527331.
9
Specific modification of fatty acid synthetase from lactating rat mammary gland by chymotrypsin and trypsin.
Biochem Biophys Res Commun. 1976 Feb 9;68(3):894-900. doi: 10.1016/0006-291x(76)91229-8.
10
Transacylation as a chain-termination mechanism in fatty acid synthesis by mammalian fatty acid synthetase. Synthesis of butyrate and hexanoate by lactating cow mammary gland fatty acid synthetase.转酰基作用作为哺乳动物脂肪酸合成酶脂肪酸合成中的链终止机制。泌乳奶牛乳腺脂肪酸合成酶合成丁酸和己酸。
Biochem J. 1980 Jan 15;186(1):287-94. doi: 10.1042/bj1860287.
引用本文的文献
1
Protein degradation in rat liver during post-natal development.大鼠肝脏在出生后发育过程中的蛋白质降解
Biochem J. 1980 Oct 15;192(1):321-30. doi: 10.1042/bj1920321.
2
Protein degradation during terminal cytodifferentiation. Studies on mammary gland in organ culture.终末细胞分化过程中的蛋白质降解。器官培养中乳腺的研究。
Biochem J. 1980 Oct 15;192(1):311-20. doi: 10.1042/bj1920311.
3
Extensive destruction of newly synthesized casein in mammary explants in organ culture.器官培养中乳腺外植体中新合成酪蛋白的广泛破坏。
Biochem J. 1982 Jan 15;202(1):133-8. doi: 10.1042/bj2020133.
4
Lipogenic enzymes in rat maternal adipose tissue in the perinatal period.围产期大鼠母体脂肪组织中的生脂酶
Biochem J. 1980 Mar 15;186(3):937-44. doi: 10.1042/bj1860937.
5
Casein turnover in rabbit mammary explants in organ culture.器官培养中兔乳腺外植体的酪蛋白周转率
Biochem J. 1979 Sep 15;182(3):837-45. doi: 10.1042/bj1820837.
本文引用的文献
1
The kinetics of catalase synthesis and destruction in vivo.过氧化氢酶在体内合成与分解的动力学。
J Biol Chem. 1962 Nov;237:3468-75.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
Characterization of radioactive immunochemically precipitated protein antigens using SDS-polyacrylamide gels.
Anal Biochem. 1971 Dec;44(2):559-69. doi: 10.1016/0003-2697(71)90245-4.
4
Human prolactin. Evidence obtained by the bioassay of human plasma.人催乳素。通过人血浆生物测定获得的证据。
J Endocrinol. 1971 Sep;51(1):157-68. doi: 10.1677/joe.0.0510157.
5
6
Intracellular protein degradation in mammalian and bacterial cells.哺乳动物细胞和细菌细胞中的细胞内蛋白质降解
Annu Rev Biochem. 1974;43(0):835-69. doi: 10.1146/annurev.bi.43.070174.004155.
7
Control of enzyme levels in animal tissues.动物组织中酶水平的调控
Annu Rev Biochem. 1970;39:929-76. doi: 10.1146/annurev.bi.39.070170.004433.
8
Influence of turnover rates on the responses of enzymes to cortisone.周转率对酶对可的松反应的影响。
Mol Pharmacol. 1965 Sep;1(2):149-56.
9
Fatty acid biosynthesis. 3. Intracellular site of enzymes in lactating-rabbit mammary gland.脂肪酸生物合成。3. 泌乳兔乳腺中酶的细胞内定位。
Biochim Biophys Acta. 1966 Dec 7;125(3):445-55.
10
Myosin synthesis and degradation during development of cardiac hypertrophy in the rabbit.兔心脏肥大发育过程中肌球蛋白的合成与降解
Circ Res. 1972 Jun;30(6):690-702. doi: 10.1161/01.res.30.6.690.
Zotero 插件