Sunshine G H, Cyrus M, Winchester G
Immunology. 1982 Feb;45(2):357-63.
In this paper we describe the first in vitro response to the liver alloantigen F. The anti-F response serves as a valuable model for autoimmune phenomena since priming appropriate strains of mice (responders) with allogeneic but not syngeneic type F leads to autoantibody production. The in vitro system is based on the proliferation of T cells, from mice primed in vivo with F, when coincubated with splenic adherent cells (SAC) prepulsed with F in vivo. The system displays two important correlates of the in vivo antibody response to F:1.T cells from mice primed with syngeneic F do not proliferate when incubated with SAC prepulsed with syngeneic F and 2. Mice that do not make antibody responses to allo F in vivo (DBA/2) do not show in vitro proliferative responses. These findings indicate that the proliferative assay is a good in vitro model for the F response.
在本文中，我们描述了对肝脏同种异体抗原F的首次体外反应。抗F反应是自身免疫现象的一个有价值的模型，因为用同种异体而非同基因的F型对合适品系的小鼠（反应者）进行致敏会导致自身抗体的产生。该体外系统基于用F在体内致敏的小鼠的T细胞与在体内用F预刺激的脾粘附细胞（SAC）共同孵育时的增殖情况。该系统显示出体内对F的抗体反应的两个重要关联：1. 用同基因F致敏的小鼠的T细胞与用同基因F预刺激的SAC孵育时不会增殖；2. 在体内对同种异体F不产生抗体反应的小鼠（DBA/2）在体外也不显示增殖反应。这些发现表明增殖试验是F反应的一个良好体外模型。
