Pistoia V, Nocera A, Ghio R, Minale P, Ferrarini M
J Natl Cancer Inst. 1982 Feb;68(2):233-7.
The colony-forming capacity of peripheral blood T-cells isolated from B-cell chronic lymphocytic leukemia patients was studied and compared with that of peripheral blood T-cells isolated from 2 groups of normal controls (young and aged healthy donors). Upon stimulation with phytohemagglutinin or pokeweed mitogen. T-cells from both the patients and the controls developed into colonies but only in the presence of the appropriate conditioned media. When the responses of the 3 groups were compared, no statistically significant differences were detected. However, the number of patients who failed to respond to phytohemagglutinin was larger than that of normal controls. Studies on a selected group of patients demonstrated that such unresponsiveness was unrelated to the presence of suppressor cells or to inadequate conditions used for stimulation in culture. Rather, this unresponsiveness may be connected with an intrinsic T-cell defect present in certain B-cell chronic lymphocytic leukemia patients.
对从B细胞慢性淋巴细胞白血病患者中分离出的外周血T细胞的集落形成能力进行了研究，并与从两组正常对照（年轻和老年健康供体）中分离出的外周血T细胞的集落形成能力进行了比较。在用植物血凝素或商陆有丝分裂原刺激后，患者和对照的T细胞都能形成集落，但前提是存在适当的条件培养基。当比较三组的反应时，未检测到统计学上的显著差异。然而，对植物血凝素无反应的患者数量多于正常对照。对一组选定患者的研究表明，这种无反应性与抑制细胞的存在或培养中用于刺激的条件不足无关。相反，这种无反应性可能与某些B细胞慢性淋巴细胞白血病患者中存在的内在T细胞缺陷有关。
