Malmström P, Jönsson A, Sjögren H O
Scand J Immunol. 1981 Nov;14(5):523-7.
Lymphocytes from various lymphoid organs of WF and BN rats were isolated and stained for acid alpha-naphthyl acetate esterase (ANAE) activity. From all organs examined-peripheral blood, lymph nodes, and spleen-70-80% of the lymphocytes were stained for ANAE activity. After separation into B and non-B cells by affinity chromatography on anti-Ig columns, esterase-negative lymphocytes were detected in both populations, demonstrating that ANAE staining cannot be used as a lymphocyte subclass marker in rats, in contrast to the situation in human and mice.
分离WF和BN大鼠各种淋巴器官中的淋巴细胞，并对其进行酸性α-萘乙酸酯酶（ANAE）活性染色。在所检查的所有器官——外周血、淋巴结和脾脏中，70%-80%的淋巴细胞被染出ANAE活性。通过抗Ig柱亲和层析将细胞分离为B细胞和非B细胞后，在这两个细胞群体中均检测到酯酶阴性淋巴细胞，这表明与人类和小鼠的情况不同，ANAE染色不能用作大鼠淋巴细胞亚类的标志物。
