Ohe Y, Hara H, Nagai K
Tissue Antigens. 1981 Aug;18(2):79-84. doi: 10.1111/j.1399-0039.1981.tb01368.x.
The human major histocompatibility antigens HLA, play an important role in transplantation. To ascertain the expression of the antigens of A and B loci on the surface of human hemopoietic progenitors, the cytotoxic effect of specific anti-HLA sera was examined. Anti-HLA A2 and B5 sera were used in the present experiments. Circulating blood mononuclear cells and nucleated marrow cells, pretreated with appropriate specific anti-HLA sera and complement, formed fewer colonies from BFU-e and CFU-c in a culture medium than the controls which were treated with autologous serum. In our experiments, the HLA antisera also killed macrophages, monocytes, and T cells which carry the surface antigen. Then, the influence of macrophages, monocytes, and T cells on colony formation from BFU-e and CFU-c was examined. These results indicated that BFU-e as well as CFU-c expressed HLA antigens on their surface.
人类主要组织相容性抗原HLA在移植中起重要作用。为确定人类造血祖细胞表面A和B位点抗原的表达情况，检测了特异性抗HLA血清的细胞毒性作用。本实验使用了抗HLA A2和B5血清。用适当的特异性抗HLA血清和补体预处理的循环血单核细胞和有核骨髓细胞，在培养基中形成的BFU-e和CFU-c集落比用自体血清处理的对照组少。在我们的实验中，HLA抗血清也杀死了携带表面抗原的巨噬细胞、单核细胞和T细胞。然后，检测了巨噬细胞、单核细胞和T细胞对BFU-e和CFU-c集落形成的影响。这些结果表明，BFU-e和CFU-c在其表面表达HLA抗原。
