Ohe Y, Hara H, Nagai K
Exp Hematol. 1982 May;10(5):467-71.
The human major histocompatibility antigen HLA, plays an important role in transplantation. To ascertain the expression of the antigens of HLA-A and -B loci on the surface of human pluripotent hemopoietic progenitors (CFUMIX), the cytotoxic effect of specific anti-HLA sera was examined. Anti-HLA sera and complement formed fewer colonies from CFUMIX in a culture medium than the controls which were treated with autologous serum. In our experiments, the HLA antisera killed also T cells that carry the surface antigen. Then the influences of T cells on mixed colony formation were examined. No reduction of colony formation was observed from the cell suspension in which T cells were depleted by treatment of monoclonal antibody OKT3. These results indicated that CFUMIX expressed HLA antigens on their surface.
人类主要组织相容性抗原HLA在移植中起重要作用。为确定人类多能造血祖细胞（CFUMIX）表面HLA - A和 - B位点抗原的表达情况，检测了特异性抗HLA血清的细胞毒性作用。在培养基中，抗HLA血清和补体形成的CFUMIX集落比用自体血清处理的对照少。在我们的实验中，HLA抗血清也杀死了携带表面抗原的T细胞。然后检测了T细胞对混合集落形成的影响。用单克隆抗体OKT3处理使T细胞耗竭的细胞悬液中，未观察到集落形成减少。这些结果表明CFUMIX在其表面表达HLA抗原。
