Harris J E, DeBoer K P, Vahey A L, Braun D P
Med Pediatr Oncol. 1982;10(2):185-94. doi: 10.1002/mpo.2950100214.
Monocyte and lymphocyte subsets were quantitated in the peripheral blood of normal subjects and patients with solid tumors using the monoclonal antibody reagents OKM1, BRL63D3, OKT3, OKT4, and OKT8. Percentages and numbers of cells reacting with monoclonal reagents were analyzed by indirect immunofluorescence. Correlations between leukocyte subset values and stage of disease or immunocompetence were sought. No differences from normal were seen in the percentage of OKT3, OKT4, and OKT8 cells or in the ratios of OKT4/OKT8 cells in peripheral blood mononuclear cells (PBMC) from all cancer patients, patients with localized disease, or patients with advanced disease. A significant decrease in absolute numbers of lymphocytes, OKT3 cells, OKT4 cells, and OKT8 cells was seen in the peripheral blood of patients with advanced disease reflecting the absolute lymphopenia of these patients. A significant increase was seen in the percentages of PBMC reacting with OKM1 and BRL63D3 from patients with advanced disease compared with normal donors or localized disease patients. A positive correlation was found between PHA responsiveness and absolute numbers of OKT3 and OKT4 cells. A negative correlation was found between PHA responsiveness and percentages of OKM1 cells. These data indicate that malignant disease does not alter T cell subset percentages in patient peripheral blood but may decrease their absolute numbers in association with absolute lymphopenia. On the other hand, percentages of OKM1 and BRL63D3 cells are increased in patients with advanced solid tumors in association with impaired PHA responsiveness.
使用单克隆抗体试剂OKM1、BRL63D3、OKT3、OKT4和OKT8对正常受试者和实体瘤患者外周血中的单核细胞和淋巴细胞亚群进行定量分析。通过间接免疫荧光分析与单克隆试剂反应的细胞百分比和数量。研究白细胞亚群值与疾病分期或免疫能力之间的相关性。在所有癌症患者、局限性疾病患者或晚期疾病患者的外周血单核细胞（PBMC）中，OKT3、OKT4和OKT8细胞的百分比或OKT4/OKT8细胞的比例与正常情况无差异。晚期疾病患者外周血中淋巴细胞、OKT3细胞、OKT4细胞和OKT8细胞的绝对数量显著减少，反映出这些患者存在绝对淋巴细胞减少症。与正常供体或局限性疾病患者相比，晚期疾病患者中与OKM1和BRL63D3反应的PBMC百分比显著增加。发现PHA反应性与OKT3和OKT4细胞的绝对数量呈正相关。发现PHA反应性与OKM1细胞的百分比呈负相关。这些数据表明，恶性疾病不会改变患者外周血中T细胞亚群的百分比，但可能会使其绝对数量减少并伴有绝对淋巴细胞减少症。另一方面，晚期实体瘤患者中OKM1和BRL63D3细胞的百分比增加，同时PHA反应性受损。
