Issekutz T B, Chin W, Hay J B
Immunology. 1982 May;46(1):59-66.
Afferent lymphatics draining Freund's adjuvant-induced granulomas and efferent lymphatics from normal subcutaneous lymph nodes were cannulated in sheep. It was previously reported that cells collected from these lymphatics, after being radiolabelled with 111In and returned to the animal intravenously, migrated from the blood back through the granuloma or lymph node into the lymph compartment from which they were originally obtained. Afferent lymph cells preferentially migrated out of the circulation in the granuloma rather than the lymph node. The cell responsible for this selective migration was found to be a small recirculating T lymphocyte. Macrophages and lymphoblasts did not demonstrate this migration. Similarly, B cells did not contribute to the lymphocyte migration observed. The migration of lymphocytes through normal uninflamed skin was examined. Afferent cells migrated through normal skin in the same way as through a granuloma, suggesting that neither antigen nor local inflammatory changes were responsible for this migration.
在绵羊身上,将引流弗氏佐剂诱导肉芽肿的输入淋巴管以及正常皮下淋巴结的输出淋巴管进行插管。此前有报道称,从这些淋巴管收集的细胞,在用铟 - 111进行放射性标记并静脉回输到动物体内后,会从血液中迁移回原来获取它们的肉芽肿或淋巴结,再进入淋巴腔室。输入淋巴细胞优先从肉芽肿而非淋巴结的循环中迁移出来。发现负责这种选择性迁移的细胞是一种小的再循环T淋巴细胞。巨噬细胞和成淋巴细胞未表现出这种迁移。同样,B细胞也未参与观察到的淋巴细胞迁移。对淋巴细胞通过正常未发炎皮肤的迁移进行了检查。输入细胞通过正常皮肤的迁移方式与通过肉芽肿的方式相同,这表明这种迁移既不是由抗原也不是由局部炎症变化引起的。