Chan G L, Little J B
Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Apr;41(4):359-68. doi: 10.1080/09553008214550411.
Labelling index data showed that in AG1518 cells, a diploid human fibroblast strain, there was a lag period of at least 14 hours between subculture from the density-inhibited plateau phase of growth and entry into DNA synthesis. Cells irradiated with 254 nm wavelength U.V. light 8 hours after subculture did not exhibit the same degree of resistance as cells irradiated in plateau phase and subcultured immediately. When cells were arrested from proliferation by maintenance in an arginine and glutamine deficient medium for 72 hours, they were nearly as resistant to U.V. light as plateau phase cells although maintenance in this medium for 24 hours after irradiation supported further recovery only after low U.V. doses. One strain of Cockayne syndrome fibroblasts was found to be resistant to U.V light in plateau phase while another strain was found to have the same survival response whether it was irradiated in the plateau or log phase of growth.
标记指数数据显示，在二倍体人类成纤维细胞系AG1518细胞中，从生长的密度抑制平台期传代培养至进入DNA合成之间存在至少14小时的延迟期。传代培养8小时后用254纳米波长的紫外线照射的细胞，其抗性程度与在平台期照射并立即传代培养的细胞不同。当细胞通过在精氨酸和谷氨酰胺缺乏的培养基中维持72小时而停止增殖时，它们对紫外线的抗性几乎与平台期细胞相同，尽管照射后在该培养基中维持24小时仅在低紫外线剂量后才支持进一步恢复。发现一株科凯恩综合征成纤维细胞在平台期对紫外线有抗性，而另一株细胞无论在生长的平台期还是对数期接受照射，其存活反应都相同。
