T和B淋巴细胞激活过程中核质关系受限
Restricted nucleocytoplasmic relationship in activation of T and B lymphocytes.
作者信息
Watanabe T, Eda Y, Ohara J
出版信息
J Exp Med. 1982 Jul 1;156(1):312-7. doi: 10.1084/jem.156.1.312.
Nuclei of murine T lymphocytes or B lymphocytes were purified and transferred into lethally irradiated whole spleen cells or B or T lymphocytes by means of polyethyleneglycol-mediated cell fusion. Transfer of lymphocyte nuclei could save the irradiated cells from cell death, and such reconstituted cells could respond to mitogens. The present study showed that nuclei of T cells could be activated in the concanavalin A-stimulated T cell cytoplasms but not in the lipopolysaccharide-stimulated B cell cytoplasms. On the other hand, nuclei of B cells were activated in the lipopolysaccharide-stimulated B cells but not in the concanavalin A-stimulated T cell cytoplasms. These data suggested that a specific interaction between cytoplasm and nucleus might exist in the activation of nuclei of each lymphocyte subset.
通过聚乙二醇介导的细胞融合，纯化小鼠T淋巴细胞或B淋巴细胞的细胞核，并将其转移到经致死剂量照射的全脾细胞或B淋巴细胞或T淋巴细胞中。淋巴细胞细胞核的转移可使受照射细胞免于细胞死亡，且这种重构细胞能对有丝分裂原产生反应。本研究表明，T细胞核可在伴刀豆球蛋白A刺激的T细胞胞质中被激活，但在脂多糖刺激的B细胞胞质中则不能。另一方面，B细胞核在脂多糖刺激的B细胞中被激活，但在伴刀豆球蛋白A刺激的T细胞胞质中则不能。这些数据表明，在每个淋巴细胞亚群细胞核的激活过程中，细胞质与细胞核之间可能存在特异性相互作用。
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