Kano Y, Sakamoto S, Kasahara T, Kusumoto K, Hida K, Suda K, Ozawa K, Miura Y, Takaku F
Cancer Res. 1982 Aug;42(8):3090-2.
Normal and malignant hematopoietic cell lines, with the exception of MOLT-3, were unable to grow in the methionine-depleted medium which was supplemented with homocysteine. Normal lymphocytes were less stimulated by mitogens in the medium containing homocysteine. Colony-forming units in culture from normal bone marrow cells were not formed in the medium in which homocysteine was substituted for methionine. These data suggest that there are no differences in the methionine requirement for cell growth between normal and malignant hematopoietic cells.
除MOLT - 3外，正常和恶性造血细胞系均无法在添加了同型半胱氨酸的蛋氨酸缺乏培养基中生长。在含有同型半胱氨酸的培养基中，正常淋巴细胞受有丝分裂原的刺激较小。用同型半胱氨酸替代蛋氨酸的培养基中不能形成来自正常骨髓细胞的培养集落形成单位。这些数据表明，正常和恶性造血细胞在细胞生长对蛋氨酸的需求上没有差异。
