Kreis W, Baker A, Ryan V, Bertasso A
Cancer Res. 1980 Mar;40(3):634-41.
Human embryonic lung fibroblasts (F-136-35-56) capable of growing in medium containing DL-homocysteine instead of L-methionine and human acute lymphoblastic leukemia cells (CCRF-HSB-2) with absolute methionine requirement exhibited dose-dependent growth inhibition when semipurified L-methionine-degrading enzyme (L-methioninase, EC 4.4.1.11) was added to the tissue cultures. When D-homocystine was added to the cultures together with L-methioninase (0.1 units/ml, which completely degraded the available L-methionine in tissue culture), the F-136-35-56 cells continued to grow whereas the CCRF-HSB-2 cells were completely inhibited. In mixed cultures of the two cell lines with added L-methioninase + D-homocystine or L-methioninase + L-homocysteine thiolactone, the normal fibroblasts grew and synthesized DNA vigorously, whereas the lymphocytic malignant cells lost their viability completely and died within 3 to 4 days.
能够在含有DL-高半胱氨酸而非L-甲硫氨酸的培养基中生长的人胚肺成纤维细胞（F-136-35-56）以及对甲硫氨酸有绝对需求的人急性淋巴细胞白血病细胞（CCRF-HSB-2），当向组织培养物中添加半纯化的L-甲硫氨酸降解酶（L-甲硫氨酸酶，EC 4.4.1.11）时，表现出剂量依赖性生长抑制。当将D-高胱氨酸与L-甲硫氨酸酶（0.1单位/毫升，可完全降解组织培养物中可用的L-甲硫氨酸）一起添加到培养物中时，F-136-35-56细胞继续生长，而CCRF-HSB-2细胞则被完全抑制。在添加了L-甲硫氨酸酶 + D-高胱氨酸或L-甲硫氨酸酶 + L-高半胱氨酸硫内酯的两种细胞系混合培养物中，正常成纤维细胞旺盛生长并合成DNA，而淋巴细胞恶性细胞则完全丧失活力并在3至4天内死亡。
