Bradley N J, Bloom H J, Davies A J, Swift S M
Br J Cancer. 1978 Aug;38(2):263-72. doi: 10.1038/bjc.1978.197.
Thirteen gliomas from 55 neurosurgical specimens, derived from 25 adults and 30 children, have been successfully grown as subcutaneous xenografts in immune-deprived or nude mice. Only 2 of the 30 paediatric specimens implanted (6.7%), a medulloblastoma and an astrocytoma Grade III, have grown compared with 11 of the 25 adult specimen (44%) which were mostly astrocytomas Grade III. Tumour growth usually occurred several months after implantation, and karyotypic analysis confirmed their human origin in all cases. The histopathology of xenografted tumours correlated with the original surgical material, both after initial implantation and when tumours had been passaged several times. Observations on tumour growth in various types of immune-deprived mice indicated that, within certain limits, the immunological competence of the host mouse did not relate to take rates of primary implants, but could affect the take rate of passaged tumours.
从55例神经外科手术标本（其中25例来自成人，30例来自儿童）中获取的13个胶质瘤，已成功在免疫缺陷或裸鼠体内生长为皮下异种移植物。在植入的30例儿科标本中，只有2例（6.7%）生长，分别为1例髓母细胞瘤和1例III级星形细胞瘤；相比之下，25例成人标本中有11例（44%）生长，大多为III级星形细胞瘤。肿瘤生长通常在植入数月后发生，核型分析在所有病例中均证实其来源于人类。异种移植肿瘤的组织病理学与原始手术材料相关，无论是在初次植入后还是肿瘤传代多次后。对各种类型免疫缺陷小鼠肿瘤生长的观察表明，在一定范围内，宿主小鼠的免疫能力与初次植入的成功率无关，但会影响传代肿瘤的成功率。
