Kniazeva G D, Sheremet'eva G F, Martynov A A
Kardiologiia. 1982;22(6):18-23.
On the material from 96 puncture biopsies of the left ventricle performed on 32 patients with chronic ischaemic heart disease during aorto-coronary shunting under extracorporeal circulation with combined cold and pharmacological cardioplegia lasting from 15 to 92 min the ultrastructure of the myocardium has been studied at the 30th-40th min of the restorative period. After restoration of cardiac activity progression of changes in cardiomyocytes was recorded, more clearly seen in the quantitative stereological analysis of the membranous components of mitochondria, the degree of damage depending on the duration of cardioplegia. Statistically significant (p less than 0.02) decrease of the area of external membranes of mitochondria at the 30th min of the restorative period was detected in patients after cardioplegia exceeding 60 min. Such changes are due, according to the authors, both to enhanced demands of energy supply to cardiomyocytes during the overloads of the restorative period and to the disorders of the microcirculation and of the rheological properties of the blood during reperfusion.
在32例慢性缺血性心脏病患者进行体外循环下主动脉 - 冠状动脉分流术期间，采用冷和药物联合心脏停搏法持续15至92分钟，对其左心室进行96次穿刺活检取材，在恢复期第30至40分钟研究了心肌的超微结构。心脏活动恢复后，记录了心肌细胞变化的进展情况，在线粒体膜成分的定量立体分析中更清晰可见，损伤程度取决于心脏停搏的持续时间。在心脏停搏超过60分钟的患者中，在恢复期第30分钟检测到线粒体外膜面积有统计学意义（p小于0.02）的减少。据作者称，这些变化既是由于恢复期过载期间心肌细胞对能量供应的需求增加，也是由于再灌注期间微循环和血液流变学特性的紊乱。
