Vetter J M, Helwig J J, Engelmann A
Ann Pathol. 1982;2(3):247-50.
T lymphocytes may be identified in the blood and in suspensions derived from lymphoid tissue, by their tendency to form rosettes with sheeps' red blood cells. Some authors have described a method of obtaining the E rosettes in histological sections (Whiteside and Rowlands, 1977; Brubaker and Whiteside, 1977, 1979; Sakamoto and Oda, 1979). We demonstrate that it is possible to fix fragments of the membrane of sheep's red blood cells obtained by thermal shock. This fixation may be demonstrated by fluorescent or electron microscopy. It is possible to demonstrate T lymphocytes in sections by using membrane from fragmented sheeps' red blood cells.
通过T淋巴细胞与绵羊红细胞形成玫瑰花结的倾向，可以在血液和源自淋巴组织的悬液中识别出T淋巴细胞。一些作者描述了在组织学切片中获得E玫瑰花结的方法（怀特赛德和罗兰兹，1977年；布鲁贝克和怀特赛德，1977年、1979年；坂本和小田，1979年）。我们证明，通过热休克获得的绵羊红细胞膜片段是可以固定的。这种固定可以通过荧光显微镜或电子显微镜观察到。使用破碎的绵羊红细胞膜可以在切片中显示T淋巴细胞。
