Ovadia H, Paterson P Y
Clin Exp Immunol. 1982 Aug;49(2):386-92.
Daily administration of indomethacin (5 mg/kg) to Lewis rats from day 7 through day 21 following sensitization to guinea-pig spinal cord neural antigen plus Freund's complete adjuvant (FCA) resulted in an aggravation of clinical signs of EAE accompanied by a higher mortality. In contrast, lymph node cells (LNC) from sensitized and drug-treated donor rats showed decreased EAE transfer activity. The in vitro Con A response of LNC from sensitized, indomethacin-treated rats was comparable to those of normal LNC or LNC from sensitized animals only. The spleen cell response of sensitized rats was significantly lower than the response seen in normal animals, but was returned to normal levels in sensitized, indomethacin-treated animals. We believe these observations reflect an indomethacin-mediated diminished accumulation of EAE effector cells within peripheral lymph node tissues together with abrogation of splenic suppressor cell activity by indomethacin-mediated inhibition of prostaglandin (PG) synthesis in Lewis rats sensitized and treated with this drug under the conditions defined.
在对豚鼠脊髓神经抗原加弗氏完全佐剂（FCA）致敏后的第7天至第21天，每天给Lewis大鼠腹腔注射吲哚美辛（5毫克/千克），结果导致实验性自身免疫性脑脊髓炎（EAE）的临床症状加重，死亡率更高。相比之下，来自致敏且经药物处理的供体大鼠的淋巴结细胞（LNC）表现出降低的EAE转移活性。来自致敏且经吲哚美辛处理的大鼠的LNC的体外刀豆蛋白A反应与正常LNC或仅来自致敏动物的LNC相当。致敏大鼠的脾细胞反应明显低于正常动物的反应，但在致敏且经吲哚美辛处理的动物中恢复到正常水平。我们认为，这些观察结果反映了在所述条件下，用该药物致敏并治疗的Lewis大鼠中，吲哚美辛介导的外周淋巴结组织内EAE效应细胞积累减少，以及吲哚美辛介导的前列腺素（PG）合成抑制导致脾抑制细胞活性的消除。
