Stenberg A, Skett P, Gustafsson J A
Biochim Biophys Acta. 1978 Sep 28;530(3):412-9. doi: 10.1016/0005-2760(78)90161-3.
Hepatocytes from adult rats were isolated and cultivated as primary monolayers for 3 days in a medium containing only 1% homologous serum. The metabolism of 4-androstene-3,17-dione was followed in hepatocytes from male, female and hypophysectomized animals. It was found that immediately after preparation, 5alpha-reductase and 16alpha-hydroxylase activities were present in the cells in amounts comparable to those found in microsomal preparations from liver homogenates. After 3 days in culture, these enzyme activities had decreased to about half the values measured on day 0. During this time the sexual differences in steroid metabolism in the cells were stable, i.e. there was no detectable induction of 16alpha-hydroxylase in cells from female animals, and the relative sex difference in 5alpha-reductase activity persisted.
从成年大鼠分离出肝细胞，并在仅含1%同源血清的培养基中作为原代单层培养3天。对雄性、雌性和垂体切除动物的肝细胞中4-雄烯-3,17-二酮的代谢进行了追踪。结果发现，制备后立即，细胞中5α-还原酶和16α-羟化酶的活性与从肝脏匀浆的微粒体制备物中发现的活性相当。培养3天后，这些酶的活性降至第0天测量值的约一半。在此期间，细胞中类固醇代谢的性别差异保持稳定，即雌性动物细胞中未检测到16α-羟化酶的诱导，5α-还原酶活性的相对性别差异持续存在。
