锇标记的多核苷酸：四氧化锇与聚-1,N6-乙烯腺苷酸的反应

Osmium-labeled polynucleotides: reaction of osmium tetraoxide, with poly-1,N6-ethenoadenylic acid.

作者信息

Marzilli L G, Hanson B E, Kapili L, Rose S D, Beer M

出版信息

Bioinorg Chem. 1978 Jun;8(6):531-4. doi: 10.1016/0006-3061(78)80006-4.

Abstract

Osmium tetraoxide, in the presence of ligands such as pyridine and bipyridine, adds across the etheno bridge of 1,N6-etheno-9-methyladenine and poly-1,N6-ethenoadenylic acid. The Os:P ratio in the labeled polynucleotide was approximately equal to 1 when bipyridine was used as the stabilizing ligand. A similar study with polycytidylic acid, which had been partially modified with chloroacetaldehyde so that some bases were converted to 3,N4-ethenocytosine, gave an OS:P ratio of approximately equal to 1.3. Calf-thymus DNA, in which the adenine and cytosine bases were modified by chloroacetaldehyde, gave an Os:P ratio of approximately equal to 1 after 24 h. These results suggest that 3,N4-ethenocytosine will add two Os labels.

摘要

在吡啶和联吡啶等配体存在的情况下，四氧化锇会加成到1,N6-乙撑-9-甲基腺嘌呤和聚1,N6-乙撑腺苷酸的乙烯桥两端。当使用联吡啶作为稳定配体时，标记的多核苷酸中锇与磷的比例约等于1。对聚胞苷酸进行的类似研究中，聚胞苷酸已用氯乙醛进行了部分修饰，使得一些碱基转化为3,N4-乙撑胞嘧啶，其锇与磷的比例约等于1.3。在小牛胸腺DNA中，腺嘌呤和胞嘧啶碱基经氯乙醛修饰后，24小时后的锇与磷的比例约等于1。这些结果表明，3,N4-乙撑胞嘧啶会添加两个锇标记。