锇标记的多核苷酸。四氧化锇在叔氮供体配体存在下与脱氧核糖核酸及合成多核苷酸的反应。

Osmium-labeled polynucleotides. The reaction of osmium tetroxide with deoxyribonucleic acid and synthetic polynucleotides in the presence of tertiary nitrogen donor ligands.

作者信息

Chang C H, Beer M, Marzilli L G

出版信息

Biochemistry. 1977 Jan 11;16(1):33-8. doi: 10.1021/bi00620a006.

Abstract

Osmium tetroxide in the presence of pyridine or 2,2'-bipyridine has been found to react completely with the pyrimidine moieties (thymine, uracil, and cytosine) in polynucleotides. Pyrimidine osmate ester moieties, L2OSO4-pyrimidine, were formed. The OSO4 has added across the 5,6 double bond and L=pyridine or 1/2-bipyridine. The pyridine derivatives were not stable and decomposed slowly after the OSO4-pyridine reagent was removed by gel chromatography. Labeled poly(uridylic acid) lost osmium completely during gel chromatography unless the eluent contained a high concentration of pyridine. The products formed between OSO4-bipyridine and polynucleotides were much more stable and the OS label was retained during and after gel chromatography. Both the OSO4-pyridine and OSO4-bipyridine reagents reacted more rapidly than the OSO4-CN-reagent.

摘要

已发现四氧化锇在吡啶或 2,2'-联吡啶存在下能与多核苷酸中的嘧啶部分（胸腺嘧啶、尿嘧啶和胞嘧啶）完全反应。形成了嘧啶锇酸酯部分，即 L₂OSO₄ - 嘧啶。OSO₄ 加成到 5,6 双键上，且 L = 吡啶或 1/2 - 联吡啶。吡啶衍生物不稳定，在用凝胶色谱法除去 OSO₄ - 吡啶试剂后会缓慢分解。标记的聚尿苷酸在凝胶色谱过程中会完全失去锇，除非洗脱液中含有高浓度的吡啶。OSO₄ - 联吡啶与多核苷酸形成的产物更稳定，并且在凝胶色谱过程中及之后 OS 标记都能保留。OSO₄ - 吡啶和 OSO₄ - 联吡啶试剂比 OSO₄ - CN - 试剂反应更快。