Bedson H S
Bull World Health Organ. 1982;60(3):377-80.
The thymidine kinase produced in cell cultures by several orthopoxviruses has been tested for sensitivity to inhibition by thymidine triphosphate (TTP). Thymidine kinase preparations from eleven variola isolates and from five "whitepox" isolates were all found to be sensitive to inhibition by TTP. Preparations from other orthopoxviruses, including vaccinia, cowpox, and monkeypox, were all resistant to inhibition by TTP. There was no overlap between the two groups of results and no viruses were found which gave intermediate results. Tests with hybrid viruses showed that this character segregated independently of other biological marker characters and is thus a valuable additional marker for variola and variola-related viruses.
几种正痘病毒在细胞培养物中产生的胸苷激酶已被测试对三磷酸胸苷（TTP）抑制作用的敏感性。来自11株天花分离株和5株“白痘”分离株的胸苷激酶制剂均被发现对TTP的抑制敏感。来自其他正痘病毒，包括牛痘、痘苗和猴痘的制剂均对TTP的抑制有抗性。两组结果之间没有重叠，也未发现产生中间结果的病毒。对杂交病毒的测试表明，这一特性与其他生物学标记特性独立分离，因此是天花和天花相关病毒的一个有价值的附加标记。
