Giroux E, DaGue B, Prakash N J
Bioinorg Chem. 1978 Sep;9(3):205-16. doi: 10.1016/s0006-3061(78)80004-0.
Intraperitoneal injections of alpha-mercapto-beta-(2-furyl)acrylic acid (MFA) induced incorporation of zinc into a low-molecular-weight metalloprotein fraction in rat-liver cytosol. Cytosol of control rats contained little or none of this material. alpha-Mercapto-beta-(2-thienyl)acrylic acid induced less incorporation of zinc in this fraction than did MFA. Twenty-two hours after administration of 65ZnSO4 (50 mumol/kg) and MFA (200 mumol/kg), serum concentrations of both total and radioisotopic zinc were markedly elevated, compared to serum concentrations in rats administered only 65ZnSO4. Further, in livers of MFA-treated rats, radioisotopic zinc concentration was significantly greater in all cytosol zinc-containing proteins resolved by gel chromatography, but total zinc was elevated only in the metallothionein fraction and not in proteins of higher molecular weight. This appears to be the first example of induction of a metallothionein-like protein by a procedure not involving food restriction or administration of metal salts.
腹腔注射α-巯基-β-(2-呋喃基)丙烯酸（MFA）可促使锌掺入大鼠肝脏胞液中的低分子量金属蛋白部分。对照大鼠的胞液中几乎不含或完全不含这种物质。α-巯基-β-(2-噻吩基)丙烯酸在该部分诱导的锌掺入量比MFA少。给予65ZnSO4（50 μmol/kg）和MFA（200 μmol/kg）22小时后，与仅给予65ZnSO4的大鼠相比，总锌和放射性同位素锌的血清浓度均显著升高。此外，在MFA处理的大鼠肝脏中，通过凝胶色谱分离的所有含锌胞液蛋白中的放射性同位素锌浓度均显著更高，但总锌仅在金属硫蛋白部分升高，而在高分子量蛋白中未升高。这似乎是通过不涉及食物限制或金属盐给药的方法诱导类金属硫蛋白样蛋白的首个实例。
