3-羟基脯氨酸的一种新制备方法及其某些性质
A new method of preparation and some properties of 3-hydroxyproline.
作者信息
Szymanovicz G, Mercier O, Randoux A
出版信息
Biochimie. 1978 Sep 4;60(5):499-503. doi: 10.1016/s0300-9084(78)80865-7.
The hydrolyzate of Delonix Regia seed extract is fractionated sequentially on Dowex 50 X 8 resin and on QAE Sephadex A 25. Purification is completed by recrystallisation from ethyl alcohol. 3-hydroxyproline is destroyed by NO2H and by chloramine T, which prevents from using most of the colorimetric reactions in use for 4-hydroxyproline. For its characterization, 3-hydroxyproline may be clearly separated from 4-hydroxyproline by TLC chromatography and by high voltage paper electrophoresis.
凤凰木种子提取物的水解产物依次在Dowex 50 X 8树脂和QAE Sephadex A 25上进行分级分离。通过从乙醇中重结晶完成纯化。3-羟基脯氨酸会被亚硝酸和氯胺T破坏，这使得大多数用于检测4-羟基脯氨酸的比色反应无法使用。为了对其进行表征，3-羟基脯氨酸可通过薄层层析色谱法和高压纸电泳法与4-羟基脯氨酸清晰分离。
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