Mondelli M, Vergani G M, Alberti A, Vergani D, Portmann B, Eddleston A L, Williams R
J Immunol. 1982 Dec;129(6):2773-8.
Peripheral blood T lymphocytes from 21 patients with chronic HBV infection were incubated with autologous hepatocytes in a microcytotoxicity assay. Cytotoxicity was significantly increased in 13 cases, and in 12 of these the cytotoxic effect of the T lymphocytes was inhibited by preincubating the liver cells with IgG containing antibodies to the hepatitis B core antigen (HBcAg). Normal human IgG and IgG containing antibodies to the hepatitis B surface antigen (HBsAG) were without effect. Control experiments using autologous fibroblasts as target cells showed low levels of T cell cytotoxicity and no blocking effect of anti-core antibody. All patients in whom it was possible to demonstrate HBcAg in liver tissue had significantly increased T cell cytotoxicity to autologous hepatocytes. These studies suggest that T cell cytotoxicity in patients with chronic HBV infection is directed against determinants resembling the hepatitis B core antigen on the plasma membrane of hepatocytes.
在微细胞毒性试验中，将21例慢性乙肝病毒感染患者的外周血T淋巴细胞与自体肝细胞一起孵育。13例患者的细胞毒性显著增加，其中12例患者的T淋巴细胞的细胞毒性作用可通过用含乙肝核心抗原（HBcAg）抗体的IgG预先孵育肝细胞来抑制。正常人IgG和含乙肝表面抗原（HBsAG）抗体的IgG则无此作用。以自体成纤维细胞作为靶细胞的对照实验显示T细胞细胞毒性水平较低，且抗核心抗体无阻断作用。在肝组织中能够检测到HBcAg的所有患者，其对自体肝细胞的T细胞细胞毒性均显著增加。这些研究表明，慢性乙肝病毒感染患者的T细胞细胞毒性是针对肝细胞质膜上类似于乙肝核心抗原的决定簇。
