[Changes in activities of DNA-dependent RNA-polymerases from rat liver under influence of glycerol, dimethyl sulfoxide and polyethylene glycol-400].

Effects of glycerol, dimethyl sulfoxide (DMSO) and polyethylene glycol (PEG) 400 on transcription of isolated nuclei and DNA in rat liver under the action of homologous RNA-polymerases I and II were studied. Addition of these organic compounds to the reaction mixture before initiation altered RNA synthesis on native DNA matrix. Addition of glycerol or DMSO to the synthesising system 1 and 5 minutes after initiation of RNA synthesis had no effect on the rate of transcription. PEG-400 inhibited incorporation of 3H-UTP into newly-synthesized independently of the time of its introduction to the reaction mixture. The data obtained suggest that the compounds under study may impair the formation of initiation complex of RNA polymerase with DNA and alter RNA synthesis in isolated nuclei of rat liver.