Bezanilla F, Taylor R E
Biophys J. 1978 Sep;23(3):479-84. doi: 10.1016/S0006-3495(78)85464-2.
The effects of temperature (3 degrees-26 degrees C) on the nonlinear components of the displacement current were measured in internally perfused, voltage clamped squid axons. Steps of potential were applied from a holding potential of -70mV (outside ground) to values from -130 to +70mV and either the current or its integral (charge) was recorded as a function of time. For that component of the charge movement not linearly related to voltage, the total charge moved in a few milliseconds (about 1,500 electronic charges/micron2) between saturation limits (e.g. -100mV to +50mV) showed an apparent increase of 13 +/- 5% for a 10 degrees C rise in temperature. Attempts to fit the falling phase of the gating current (or charge) with the sum of two exponentials showed temperature effects on both components but there was considerable scattering. At short times, records for current or charge made at 16 degrees C, expanded by a factor alpha, superimposed on those made at 6 degrees C for alpha about 1.6. For long times alpha was about 2.3.
在内部灌注、电压钳制的枪乌贼轴突中，测量了温度（3摄氏度至26摄氏度）对位移电流非线性成分的影响。从-70mV（外接地）的保持电位施加电位阶跃至-130mV至+70mV的值，并记录电流或其积分（电荷）随时间的变化。对于电荷移动中与电压非线性相关的成分，在饱和极限（如-100mV至+50mV）之间几毫秒内移动的总电荷（约1500个电子电荷/微米²）显示，温度每升高10摄氏度，明显增加13±5%。用两个指数之和拟合门控电流（或电荷）的下降阶段的尝试表明，温度对两个成分都有影响，但存在相当大的离散。在短时间内，16摄氏度下记录的电流或电荷记录，放大系数为α，叠加在6摄氏度下记录的记录上，α约为1.6。在长时间内，α约为2.3。
