Mycolase II: an enzyme antifungal agent interacts with the polyene antibiotic pimaricin in the treatment of keratomycosis.

An animal model of keratomycosis was used to study the interaction between the new enzyme antifungal compound mycolase II and the polyene antifungal antibiotic pimaricin. Well established corneal infection caused by an ocular pathogenic candida albicans on New Zealand white male rabbits were treated with 3% mycolase II, 5% pimaricin and a combination of 3% mycolase II and 5% pimaricin respectively. The rates of resolution of the corneal lesions for each group of eyes treated by the various drugs were determined and the results were analysed by computer using a two-way analysis of variance to determine the interaction or independence of 3% mycolase II in combination therapy with 5% pimaricin in rabbit keratomycosis. The analysis of variance showed a significant level of positive interaction after each period of treatment. (P less than 0.001).